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8 Comments to C-Note And Grants (Accapella)

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    4th Quarter ‎– C-Note And Grants C-Note And Grants (Accapella) Credits Engineer – Darrell Alston; Producer – Karl "Nut" Hampton* Notes Published by Scojo Music (BMI) Barcode and Other Identifiers Barcode: 0 8 4th Quarter: C-Note And Grants /5(4).
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    Wash cells with 1 ml of 1% BSA in PBS three times. Fix the cells again with ml of 70% ethanol O/N at 4 o C. Note: Ethanol fixing is critical to quench the internal GFP/RFP fluorescence due to the recombinant-tagged protein expression. Centrifuge the cells again at x g for 5 min and wash the pellet with 1 ml of 1% BSA in PBS once.
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    Single cell preparation. Single cells were separated from undifferentiated calli derived from A. thaliana Wassilewskija (WS-2) 35S GFP-MBD (green fluorescent protein microtubule binding domain marker) plants ().Calli were grown on g/L of MSARI-modified medium (Murashige & Skoog media with vitamins, Cat. No. M), 30 g/L of sucrose (Cat. No. S), KOH, and plant agar (Cat. No. P

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